Publications - JLRG

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Publications
1. J.L. Lee and R.E. Levin. Detection of 5 CFU/g of Escherichia coli O157:H7 on lettuce using activated charcoal and real-time
    PCR without enrichment. 2011. Food Microbiology. 2011. 28: 562-567.
2. J.L. Lee and R.E. Levin. Selective detection of mixed bacterial survivors from fish fillets after freezing and thawing by ethidium bromide monoazide real-time PCR. Food Biotechnology. 2010. 24: 270-281.
3. J.L. Lee and R.E. Levin. Discrimination of viable and dead Vibrio vulnificus after refrigerated and frozen storage using EMA, sodium deoxycholate and real-time PCR. Journal of Microbiological Methods. 2009. 79: 184-188.
4. J.L. Lee and R.E. Levin. Improved detection of g-irradiated Vibrio vulnificus after heat and cold shock treatment by using ethidium monoazide real-time PCR. Food Science and Biotechnology. 2009. 18: 788-792.
5. J.L. Lee and R.E. Levin. Detection of Vibrio vulnificus before and after g-irradiation in quahog clams (Mercenaria mercenaria) tissue using real-time polymerase chain reaction. Food Biotechnology. 2009. 23: 121-132.
6. J.L. Lee and R.E. Levin. A comparative study of the ability of EMA and PMA to distinguish viable from heat killed mixed bacterial flora from fish fillets. Journal of Microbiological Methods. 2009. 76: 93-96.
7. J.L. Lee and H.Y. Kim. Expression and iron storage properties of recombinant human ferritins in Escherichia coli:Relevance for functional ferritins in food systems. Food Biotechnology. 2008. 22: 140-149.
8. J.L. Lee and R.E. Levin. New approach for discrimination of g-irradiated Vibrio vulnificus by using Real-time polymerase chain reaction. Journal of Microbiological Methods. 2008. 73: 1-6.
9. J.L. Lee and R.E. Levin. and H. Y. Kim. Improved co-expression and multi-assembly properties of recombinant human ferritins subunit in Escherichia coli. J. Microbiol. Biotechnol. 2008. 18: 926-932.
10. J.L. Lee and R.E. Levin. Discrimination of g-irradiated Vibrio vulnificus by using ethidium monoazide real-time polymerase chain reaction. Journal of Applied Microbiology. 2008. 104: 728-734.
11. J.L. Lee and R.E. Levin. Quantification of total viable bacteria on fish fillets by using ethidium monoazide Real-time polymerase chain reaction. International Journal of Food Microbiology. 2007. 118: 312-317.
12. J.L. Lee., C.S. Park., and H.Y. Kim. Functional assembly of recombinant human ferritin subunits in Pichia pastoris. J. Microbiol. Biotechnol. 2007. 17: 1695-1699.
13. J.L. Lee and R.E. Levin. Rapid quantification of total bacteria on cod fillets by using real-time PCR. Journal of Fisheries Sciences. 2007. 1: 58-67.
14. J.L. Lee and R.E. Levin. Selection of universal primer for quantification of total bacteria associated with fish fillets. Food Biotechnology. 2006. 20: 275- 285.
15. J.L. Lee and R.E. Levin. Direct application of the polymerase chain reaction for quantification of total bacteria on fish fillets. Food Biotechnology. 2006. 20: 287-298.
16. J.L. Lee and R.E. Levin. Use of ethidium bromide monoazide for quantification of viable and dead mixed bacterial flora from fish fillets by polymerase chain reaction. Journal of Microbiological Methods. 2006. 67: 456- 462.
17. J. L. Lee, S.N. Yang, C. S. Park, D. Jeoung, and H. Y. Kim.  Purification and its glycosylation pattern of recombinant L-
     ferritin in Pichia pastoris. J. Microbiol. Biotechnol. 2004. 14: 68-73.
18. J. L. Lee, H. S. Song, H, J. Kim, J. H. Park., D. K. Chung., C. S. Park., D. Jeoung., and H. Y. Kim. Functional expression and production of human H-ferritin in Pichia pastoris. Biotechnology Letters. 2003. 25:1019-1023.
19. S. N. Yang, H. S. Song., J. L. Lee and H. Y. Kim. Selection of enhanced iron uptake yeast mutants by EMS mutagenesis. Kor. J. Microbiol. Biotechnol. 2003. 31: 435-438.
20. J. L. Lee., J. H. Park., I. S. Jung., D, Jeoung., S. K. Cho., H. Y. Kim. Purification and characterization of the recombinant
     human Iron Regulatory Protein 2 expressed in Escherichia. coli. Agric. Chem.Biotechnol. 2002. 45:117-120.
21. J. L. Lee, J. H. Park and H. Y. Kim.  Identification of differentially expressed genes using RNA differential display. Agric. Chem.Biotechnol. 1999. 42: 123-127.
22. H. Y. Kim, I. S. Jung, and J. L. Lee. Regulation of ferritin synthesis in 5’-untranslated region by Iron Responsive Element.  Agric. Chem.Biotechnol. 1998. 41: 224-227.
 
 
Presentations
1.  J. L. Lee. Microbial evaluation of the freshness of cod fillet using real-time PCR for quantification of bacteria on  
     fish fillets. UKC Annual meeting. (2009, Raleigh, USA).
2. J. L. Lee and R. E. Levin. Selective detection of mixed bacterial survivors from fish fillets after freezing and thawing by ethidium bromide monoazide real-time PCR. IFT Annual meeting. (2009, Anaheim, USA).
3. J. L. Lee and R. E. Levin. Discrimination of gamma (r)-irradiated & non irradiated Vibrio vulnificus in quahog clams (Mercenaria mercenaria) using real-time PCR. IFT Annual meeting. (2008, New Orleans, USA).
4. J. L. Lee and R. E. Levin. Discrimination of g-irradiated & non irradiated Vibrio vulnificus in quahog clams (Mercenaria mercenaria) using Real-Time Polymerase Chain Reaction. Annual Atlantic Fisheries Technology Conference. (2007, Portland, USA).
5. J. L. Lee and R. E. Levin. Discrimination of g-irradiated & non r-irradiated Vibrio vulnificus using Real-Time Polymerase Chain Reaction. IFT Annual meeting. (2007, Chicago. USA).
6. J. L. Lee. Use of ethidium bromide monoazide (EMA) for quantification of viable and dead bacteria from fish fillets by PCR and real-time PCR. Cambridge Healthtech Institute’s nucleic acid-based technologies conference. (2007, Baltimore, USA).
7. J. L. Lee and R. E. Levin. Quantification of total viable bacteria on fish fillets by using PCR and EMA real-time   
    PCR. Trans-Atlantic Fisheries Technology Conference.(2006,Quebec, Canada).
8. J. L. Lee and R. E. Levin. Rapid quantification of viable total bacteria on fish fillets by using ethidium bromide
    monoazide real-time PCR. IFT Annual meeting. (2006, Orlando. USA).
9. J.L. Lee, and H. Y. Kim. Expression of recombinant human H-ferritin in Pichia pastoris. CHI Conference (2004,
    San Diego, USA).
10. J.L. Lee, and H. Y. Kim. Functional expression of human L-ferritin in Pichia pastoris. Annual- Meeting of the Korean Society for Microbiology and Biotechnology, Bio Exhibition &International Symposium. (2003, Muju, Korea).
11. J.L. Lee, and H. Y. Kim. Expression of human H-and L-ferritin in Pichia pastoris. Annual Meeting of the Korean society for microbiology and biotechnology. (2001, Fall, Sang-Rok, Korea).
12. J.L. Lee, and H. Y. Kim. The constriction of bicistronic expression vector for studying human ferritin  
      heteropolymers. Annual Meeting of the Korean Society for Applied Biological Chemistry. (2000, Spring, Young-
      Nam National Univ, Korea).
13. J.L. Lee, and H. Y. Kim. Overexpression of recombinant ferritins in E. coli. Annual Meeting of the Korean Society of Food Science & Technology. (1999, Fall, Kyung-Hee Univ, Korea).
14. J.L. Lee, and H. Y. Kim. Characterization of the recombinant Human H-L-Ferritin. Annual Meeting of the Korean Society for Applied Biological Chemistry. (1999, Spring, Kang-Won National Univ, Korea).
15. J.L. Lee, J. H. Park, and H. Y. Identification of differentially expressed genes using RNA differential display. Annual Meeting of the Korean Society for Biochemistry and Molecular Biology. (1999, Spring, A-Ju Univ, Korea).
16. J.L. Lee and H. Y. Kim. Expression and purification of human Ferritin in Escherichia. coli. Annual Meeting of
      the Korean Society for Biochemistry and Molecular Biology. (1999, Spring, A-Ju Univ, Korea).
17. J.L. Lee and H. Y. Kim. Characterization of Iron-regulated protein in iron metabolism. Annual Meeting of the
      Korean Society for Molecular and Cellular Biology. (1998, Seoul National Univ, Korea).
18. J.L. Lee and H. Y. Kim. Characterization of Nramp2 as an iron transport protein. Annual Meeting of the Korean
      Society for Biochemistry and Molecular Biology. (1998, Fall, KAIST, Korea).
19. J.L. Lee and H. Y. Kim. Structure and function of the iron responsive element. Annual Meeting of the Korean
      Society for Biochemistry and Molecular Biology. (1998, Spring, Korea Univ, Korea).
20. J.L. Lee and H. Y. Kim. Binding characterization of iron regulatory protein to RNA ligand. Annual Meeting of
      the Korean Society for Applied Biological Chemistry. (1997, Seoul National Univ, Korea).

 

 

 

Contact
Jung-lim Lee, Ph.D.
Assistant Professor in Food Microbiology and Food Safety,
Food Science Program,
Department of Human Ecology,
1200 N. DuPont Highway, Dover, DE, 19901.
Fax: (302) 857-6441
jlee@desu.edu
 

Food Microbiology Lab