DNA Core Equipment & Services

The King Fisher Duo Prime system isolates DNA, RNA, and proteins from a variety of starting materials including cell-free body fluids, blood, bacteria, cell cultures, tissue and plant samples. The automated magnetic bead technology allows excellent recovery of samples with great reproducibility and efficiency.

Qubit fluorometers detect fluorescent dyes that are specific to the sample type (DNA, RNA, and protein). The Qubit assay selectively detects DNA, RNA, and protein using UV absorbance. The assay requires only a small amount (1 μL) of sample and detects as little as 10 pg/μL of DNA. Qubit nucleic acid quantification is considered the gold standard for downstream applications such as NGS.

The BluePippin has the DNA size selection capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA.  For long-range genomic applications, high-pass filtering allows users to collect all fragments above a size thresh-old set by the user. The cassettes for DNA selections are available in four concentrations to provide a full range of size selection options between 100 bp and 50 kb.

The MiSeq System facilitates research with a wide range of sequencing applications. It is capable of automated paired-end reads up to 600 bp and produces up to 15 Gb per run. With the longest read length of any Illumina platform, it can produce up to 25 million sequencing reads. The library prep kits it uses are optimized for a variety of applications, including targeted gene sequencing, small genome sequencing, metagenomics, and plasmid sequencing.

The BioSoft Integrators High Performance Computing (HPC) cluster is a turnkey, high-performance computing solution designed specifically to meet the computing needs of next generation sequencing/bioinformatics applications. The HPC Cluster is equipped with pre-configured genetic analysis packages optimized for maximum performance. The HPC is compatible with Illumina and PacBio data, as well as other purposes of machine learning and AI.

The NGC Quest Plus system provides accurate gradients for high-resolution separations for any biological molecules. The system is equipped with a multi-wavelength detector with simultaneous four-wavelength monitoring (between 190 and 800 nm) for high-accuracy detection of proteins and peptides combined with conductivity measurements. Fractionated samples can be easily collected from analytical- to preparative-scale purifications using the fraction collector.